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Microbiology - MB3

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60 questions

Jun 10, 2025

Question 1

How can microbial growth be estimated?

Réponse

By determining cell nitrogen, cell dry weight, culture turbidity, and culture oxygen consumption.

Question 2

What is one method to estimate microbial growth?

Réponse

Determining cell nitrogen.

Question 3

What does culture turbidity indicate?

Réponse

It is one of the methods to estimate microbial growth.

Question 4

What is the significance of oxygen in obligate aerobes?

Réponse

They require the presence of O2.

Question 5

What are obligate anaerobes sensitive to?

Réponse

They are sensitive to the presence of O2.

Question 6

What enzymes are important for oxidative phosphorylation?

Réponse

Catalase, peroxidase, and superoxide dismutase.

Question 7

What is the catalase test used for?

Réponse

To differentiate aerobic and anaerobic bacteria.

Question 8

What happens to bacteria with the catalase enzyme when exposed to hydrogen peroxide?

Réponse

They are sensitive to hydrogen peroxide.

Question 9

What do facultative anaerobes require?

Réponse

They can grow in the presence or absence of O2.

Question 10

What is the role of catalase in bacteria?

Réponse

It breaks down hydrogen peroxide.

Question 11

How do obligate anaerobes react to hydrogen peroxide?

Réponse

They do not have catalase and are negative to the catalase test.

Question 12

What is a characteristic of facultative anaerobes?

Réponse

They are positive for catalase.

Question 13

What does the presence of catalase indicate?

Réponse

It indicates the ability to break down hydrogen peroxide.

Question 14

What type of bacteria does not have catalase?

Réponse

Obligate anaerobes.

Question 15

What is the effect of hydrogen peroxide on obligate anaerobes?

Réponse

They are negatively affected as they lack catalase.

Question 16

What is a common feature of obligate aerobes?

Réponse

They require oxygen for growth.

Question 17

What type of bacteria can grow with or without oxygen?

Réponse

Facultative anaerobes.

Question 18

What is the relationship between catalase and hydrogen peroxide?

Réponse

Catalase breaks down hydrogen peroxide.

Question 19

What type of bacteria is sensitive to H2O2?

Réponse

Obligate aerobes.

Question 20

What does the term 'oxidative phosphorylation' refer to?

Réponse

A process involving enzymes like catalase.

Question 21

What is the outcome of the catalase test for aerobic bacteria?

Réponse

They are positive for catalase.

Question 22

What do facultative anaerobes produce in the presence of oxygen?

Réponse

They produce catalase.

Question 23

What is a negative result in the catalase test indicative of?

Réponse

It indicates the absence of catalase, typical of obligate anaerobes.

Question 24

What is the function of superoxide dismutase?

Réponse

It helps in the detoxification of superoxide radicals.

Question 25

What type of bacteria are obligate aerobes?

Réponse

Bacteria that require oxygen.

Question 26

What do you call bacteria that can survive without oxygen?

Réponse

Facultative anaerobes.

Question 27

What is a key characteristic of obligate anaerobes?

Réponse

They cannot survive in the presence of oxygen.

Question 28

What does the presence of superoxide dismutase indicate?

Réponse

It indicates the ability to detoxify superoxide radicals.

Question 29

What is the role of peroxidase in bacteria?

Réponse

It helps in breaking down hydrogen peroxide.

Question 30

What is a defining trait of facultative anaerobes regarding oxygen?

Réponse

They can grow in both the presence and absence of oxygen.

Question 31

What is the effect of hydrogen peroxide on facultative anaerobes?

Réponse

They are generally resistant due to catalase.

Question 32

What is the significance of the catalase enzyme?

Réponse

It protects bacteria from oxidative damage.

Question 33

What type of bacteria is not affected by hydrogen peroxide?

Réponse

Facultative anaerobes due to their catalase.

Question 34

What is the outcome of the catalase test for obligate anaerobes?

Réponse

They are negative for catalase.

Question 35

What is the relationship between obligate aerobes and oxygen?

Réponse

They need oxygen to grow.

Question 36

What does the presence of oxygen indicate for obligate aerobes?

Réponse

It indicates a suitable environment for growth.

Question 37

What is the role of oxygen in microbial growth?

Réponse

It is essential for obligate aerobes.

Question 38

What is the function of the catalase enzyme in aerobic bacteria?

Réponse

It breaks down hydrogen peroxide into water and oxygen.

Question 39

What type of bacteria can tolerate the presence of oxygen?

Réponse

Facultative anaerobes.

Question 40

What is a common feature of obligate anaerobes?

Réponse

They lack the enzymes to break down hydrogen peroxide.

Question 41

What is the significance of the catalase test in microbiology?

Réponse

It helps classify bacteria as aerobic or anaerobic.

Question 42

What is the effect of hydrogen peroxide on aerobic bacteria?

Réponse

They can break it down due to catalase.

Question 43

What type of bacteria is catalase positive?

Réponse

Aerobic bacteria.

Question 44

What is the relationship between facultative anaerobes and catalase?

Réponse

They are catalase positive.

Question 45

What do obligate anaerobes lack?

Réponse

They lack catalase.

Question 46

What is the role of enzymes in microbial growth?

Réponse

They facilitate metabolic processes.

Question 47

What is a characteristic of facultative anaerobes regarding oxygen?

Réponse

They can switch between aerobic and anaerobic metabolism.

Question 48

What is the effect of oxygen on obligate anaerobes?

Réponse

It is harmful to them.

Question 49

What is the importance of chemical requirements in microbial growth?

Réponse

They are essential for metabolic functions.

Question 50

What do you call bacteria that thrive in the presence of oxygen?

Réponse

Obligate aerobes.

Question 51

What is the impact of hydrogen peroxide on facultative anaerobes?

Réponse

They can detoxify it due to catalase.

Question 52

What is the outcome for obligate anaerobes in the presence of oxygen?

Réponse

They cannot survive.

Question 53

What is the role of oxygen in oxidative phosphorylation?

Réponse

It acts as the final electron acceptor.

Question 54

What is the significance of superoxide dismutase in bacteria?

Réponse

It protects against oxidative stress.

Question 55

What do you call bacteria that can live without oxygen?

Réponse

Anaerobes.

Question 56

What is a defining feature of aerobic bacteria?

Réponse

They require oxygen for growth.

Question 57

What is the relationship between catalase and hydrogen peroxide?

Réponse

Catalase decomposes hydrogen peroxide.

Question 58

What is a common characteristic of facultative anaerobes?

Réponse

They can adapt to different oxygen levels.

Question 59

What is the effect of hydrogen peroxide on aerobic bacteria?

Réponse

They can break it down safely.

Question 60

What do obligate anaerobes do in the absence of oxygen?

Réponse

They thrive and grow.

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